The impact of polynucleotides as an injectable proliferant on the presentations of simulated post-traumatic osteoarthrosis

Relevance. Post-traumatic osteoarthrosis (PTOA) is a multifactorial disease caused by mechanical and biological changes. It mainly features the degeneration of the articular cartilage and subchondral bone, and changes in the properties of synovial fluid; its viscosity decreases which requires correction. When entering the joint, the bioactive substances consisting of polynucleotides break into simple nucleotides, nucleosides, and nucleobases, which, as is known from the published studies, are present in the extracellular environment in physiologic concentrations, and are fundamental substrates for joint cell regeneration.

The objective of this study is to investigate the impact of polynucleotides as injectable proliferants on the presentations of simulated PTOA.

Materials and methods. The experiment involved white outbred male rats. The intensity of lipid peroxidation was assessed by lipid hydroperoxides (LHP) accumulation in the serum of the animals, and antioxidant system activity by thiol status (TS). To assess the synthetic osteoblasts activity osteocalcin (OC) content was determined, and the articular cartilage metabolism was defined by hyaluronan (HN) content.

Results. On Day 7 of the experiment, the PTOA simulation was confirmed by the changes in the levels of serum markers. Resulting from intra-articular administration of polynucleotide-based proliferates on Day 28 the decrease in HPL was observed while TS was increased. The polynucleotides didn’t cause any imbalance the regulation mechanisms of the joint osteochondral component which was confirmed by a decrease in OC and HN values on Day 28 versus the comparison group.

Conclusion. The experiment findings suggest the onset of degenerative changes as early as on Day 7 of PTOA simulation. Polynucleotides injected as proliferants are substances capable of supporting physiological restoration in osteochondral structures as well as redox reactions in articular tissues in simulated PTOA.

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